Classification of heterotic groups with RFLPs among 45 maize inbred lines

Huang YiQin; Li JianSheng

Scientia Agricultura Sinica 34(3): 244-250


ISSN/ISBN: 0578-1752
Accession: 003679868

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The classification of heterotic groups is essential to maize breeding because knowledge of heterotic groups can direct combination of outstanding hybrids and improvement of elite inbred lines. RFLP has provided a powerful tool to assign maize inbred lines into heterotic groups. In this study, 45 maize inbred lines were chosen for RFLP analysis, among which 41 lines were parent lines of widely extensive hybrids in south and southwest China, and 4 lines came from America, representing different heterotic groups in US maize belt. Fifty-four RFLP core markers covering 10 chromosomes of maize were used. Total DNA was digested with EcoR I, BamH I and Hind III. The procedure of RFLP was employed as described by a manual from maize RFLP lab at University of Missouri, Colombian. A total of 860 bands were detected among 45 inbred lines based on RFLP analysis, which were involved in 212 loci. The number of alleles at each locus ranged from 2 to 9, with an average of 4.06. In total, 45 inbred lines were classified into 6 heterotic groups according to RFLP data with Ward's method. Three heterotic groups, Mo17, FRB73 and Oh43, seemed to be similar with the US heterotic groups. Twenty-one inbred lines, most of which derived from the Chinese local germplasm, were classified into two heterotic groups, indicating that the local germplasm was different from the US germplasm at the molecular level and played an important role in maize hybrid production in China. Two inbred lines from tropic germplasm were assigned in the same group. These results provided useful information to understand maize germplasm in China.