Clone and expression of the truncated envelope antigenic gene in E. coli
Clone and expression of the truncated envelope antigenic gene in E. coli
Weng YuWei; Yan YanSheng; L.S.iQing; H.S.i; X.L.ngShan
Chinese Journal of Zoonoses 19(2): 19-22
2003
Aim: To obtain the recombinant antigen used for detection of dengue virus infection. Method: The Dengue virus NGC strain, serotype 2 virus, was cultured through infected the C6/36 monolayer cell. The viral RNA was extracted from the cultured supernatant. The artificial synthesized primers were applied to amplify the viral envelope antigenic gene fragment by reverse-transcription nested PCR.