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Clone and expression of the truncated envelope antigenic gene in E. coli

Clone and expression of the truncated envelope antigenic gene in E. coli

Chinese Journal of Zoonoses 19(2): 19-22

Aim: To obtain the recombinant antigen used for detection of dengue virus infection. Method: The Dengue virus NGC strain, serotype 2 virus, was cultured through infected the C6/36 monolayer cell. The viral RNA was extracted from the cultured supernatant. The artificial synthesized primers were applied to amplify the viral envelope antigenic gene fragment by reverse-transcription nested PCR.

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