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Cloning HaSNPV iap2 gene and its expression in prokaryotic expression system

Cloning HaSNPV iap2 gene and its expression in prokaryotic expression system

Entomological Knowledge 40(1): 35-39

The iap2 gene of HaSNPV was located in the BamH1-F fragment trough. The genome of Helicoverpa armigera single capsid nuclear polyhedrosis virus and the known sequences already available from GenBank was analysed. The BamH1-F fragment was used as a template and design primer to obtain the DNA fragment of the iap2 gene by PCR amplification.

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