+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Cloning and expression analysis of hemoglobin genes from maize (Zea mays ssp. mays) and teosinte (Zea mays ssp. parviglumis)

Cloning and expression analysis of hemoglobin genes from maize (Zea mays ssp. mays) and teosinte (Zea mays ssp. parviglumis)

Biochimica et Biophysica Acta, Gene Structure and Expression 1522(1): 1-8

With the exception of barley and rice, little is known about the existence of hemoglobins (Hbs) in cereals. This work reports the cloning and analysis of hb genes from maize (Zea mays ssp. mays) and teosinte (Zea mays ssp. parviglumis). Coding sequences of maize and teosinte hb genes (hbm and hbt, respectively) are highly similar to each other and are interrupted by three introns located at identical positions as other plant hb genes. Sequences of predicted Hbm and Hbt proteins are identical. The hydropathic profile of Hbm and Hbt is highly similar to that of rice Hb1, suggesting that Hbm, Hbt and Hb1 have the same tertiary structure and biochemical properties. Expression analysis showed that low levels of Hb transcripts, but considerable levels of Hb proteins exist in maize embryonic organs. No Hb transcripts and proteins were detected in teosinte embryonic organs. Low levels of Hb proteins, but no Hb transcripts, were detected in maize and teosinte vegetative organs. These observations suggest that the regulation of hb genes is different in maize and teosinte embryonic organs, and that the expression of hb genes is down- or up-regulated in maize and teosinte, respectively, from germination to vegetative growing.

(PDF emailed within 0-6 h: $19.90)

Accession: 003681328

Download citation: RISBibTeXText

PMID: 11718894

DOI: 10.1016/s0167-4781(01)00288-3

Related references

From teosinte zea mays ssp mexicana or zea mays ssp parviglumis to maize zea mays ssp mays the catastrophic sexual transmutation. Science (Washington D C) 222(4626): 886-894, 1983

Plant defense against fall armyworm in micro-sympatric maize Zea mays ssp mays and Balsas teosinte Zea mays ssp parviglumis. Entomologia Experimentalis et Applicata 145(3), 2012

Population genetics of duplicated disease-defense genes, hm1 and hm2, in maize (Zea mays ssp. mays L.) and its wild ancestor (Zea mays ssp. parviglumis). Genetics 162(2): 851-860, 2002

Cytogenetic analysis of the hybrids Zea mays ssp. mays X Z. mays ssp. parviglumis and Z. mays ssp. mays X Z. mays ssp. mexicana. Maize Genetics Cooperation Newsletter ( 66): 60, 1992

Fine mapping of a quantitative resistance gene for gray leaf spot of maize (Zea mays L.) derived from teosinte (Z. mays ssp. parviglumis). Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 130(6): 1285-1295, 2017

Major regulatory genes in maize contribute to standing variation in teosinte (Zea mays ssp. parviglumis). Genetics 177(4): 2349-2359, 2007

Simultaneous selection for stem borer resistance and forage related traits in maize (Zea mays ssp. mays L.) teosinte (Zea mays ssp. mexicana L.) derived populations. Crop Protection 57: 27-34, 2014

Ploidy levels affect phenotype and cytogenetic traits in Zea mays ssp. mays (2n=20 or 40) and Zea mays ssp. parviglumis hybrids. Cytologia 66(2): 189-196, 2001

Relationships between Zea mays ssp. mays and Zea mays ssp. parviglumis by genomic in situ hybridization (GISH). Maize Genetics Cooperation Newsletter (75): 36, 2001

A cellular study of teosinte Zea mays subsp. parviglumis (Poaceae) caryopsis development showing several processes conserved in maize. American Journal of Botany 96(10): 1798-1807, 2009

Preliminary results of crossing inbred lines of maize (Zea mays) with teosinte (Zea mays subsp. mexicana Schradiltis). Selektsiya i semenovodstvo rannespelykh gibridov kukuruzy: Doklady nauchno metodicheskogo soveshchaniya po problemam: 71-93, 1991

Flooding tolerance in interspecific introgression lines containing chromosome segments from teosinte (Zea nicaraguensis) in maize (Zea mays subsp. mays). Annals of Botany 112(6): 1125-1139, 2014

Inheritance and chemical composition in inbred maize (Zea mays L.) X teosinte (Zea mays subsp. mexicana Schrader/ Iltis) hybrids. (Preliminary communication). Novenytermeles 39(3): 193-213, 1990

Ethnobotany of the balsas teosinte (Zea mays ssp. parviglumis). Maydica 50(2): 123-128, 2005

Cytogenetic study of diploid and triploid F1 hybrids of Zea mays ssp. mays x Zea parviglumis. Maize Genetics Cooperation Newsletter ( 71): 54-55, 1997