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Cloning and sequence analysis of the matrix protein, 5a and 5b protein genes from infectious bronchitis virus proventriculus strain isolated in Qingdao, China


Cloning and sequence analysis of the matrix protein, 5a and 5b protein genes from infectious bronchitis virus proventriculus strain isolated in Qingdao, China



Chinese Journal of Veterinary Science 22(2): 108-110



Three primers were designed and synthesized according to the reported IBV sequences in Genebank. The viral RNA of IBV proventriculus strain Qingdao strain (SD 97/02) was amplified through RT-PCR. A DNA fragment of about 1.6 kb, which spans the matrix protein (M), 5a and 5b protein genes, was amplified. Then, the PCR product was cloned and sequenced.

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