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Cloning and sequence analyzing of B.t.i cryIVD gene


Cloning and sequence analyzing of B.t.i cryIVD gene



Chinese Journal of Parasitic Disease Control 16(1): 52-54



A study was conducted to clone Bacillus thuringiensis subsp. israelensis (B.t.i.) cryIVD gene and determine its nucleotide sequence. A pair of primer was designed according to the known sequence of B.t.i. cryIVD. The B.t.i. cryIVD gene was amplified by PCR technique and cloned into pUC18 vector. The positive clones were screened and identified by agarose gel electrophoresis and endonuclease digestion to determine its nucleotide sequence.

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