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Cloning and sequence comparison of L2 genes of bluetongue virus from China


Cloning and sequence comparison of L2 genes of bluetongue virus from China



Chinese Journal of Veterinary Science 22(6): 536-538



A portion of the L2 gene from 15 field strain isolates and 1 attenuated vaccine strain of Chinese BTV serotype 1, corresponding to nucleotide 854-1806, was amplified via reverse transcription-polymerase chain reaction (RT-PCR), cloned and sequenced. Phylogenetic analysis was done on the amplified region of the L2 gene of Chinese strains of BTV serotype 1. The L2 gene sequences segregated into two distinct monophyletic groups (A and B). Group A included Y863 strain isolated in 1979.

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