Cloning and sequencing S2 gene of avian proventriculopathic infectious bronchitis virus Chinese isolate ZJ971
Cloning and sequencing S2 gene of avian proventriculopathic infectious bronchitis virus Chinese isolate ZJ971
Shen XingYan; Zhou JiYong; Ding HongMei; W.J.anXiang; Yan QingFeng
Chinese Journal of Veterinary Science 21(6): 540-542
2001
The cDNA of S2 gene of avian proventriculopathic infectious bronchitis virus (IBV) Chinese isolate ZJ971 was amplified by reverse transcription-polymerase chain recation (RT-PCR) and then cloned into vector pBluescript SK. The resulting plasmid was identified by a restriction enzyme for the S2 insert which was eventually sequenced. The sequencing data indicated that the S2 gene cloned comprised an 1872 bp nucleotides sequence encoding a 530 amino acid polypeptide.