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Cloning and sequencing S2 gene of avian proventriculopathic infectious bronchitis virus Chinese isolate ZJ971


Cloning and sequencing S2 gene of avian proventriculopathic infectious bronchitis virus Chinese isolate ZJ971



Chinese Journal of Veterinary Science 21(6): 540-542



The cDNA of S2 gene of avian proventriculopathic infectious bronchitis virus (IBV) Chinese isolate ZJ971 was amplified by reverse transcription-polymerase chain recation (RT-PCR) and then cloned into vector pBluescript SK. The resulting plasmid was identified by a restriction enzyme for the S2 insert which was eventually sequenced. The sequencing data indicated that the S2 gene cloned comprised an 1872 bp nucleotides sequence encoding a 530 amino acid polypeptide.

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