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Cloning and sequencing of a cDNA sequence encoding FAD2 from Brassica napus

Cloning and sequencing of a cDNA sequence encoding FAD2 from Brassica napus

Journal of Hunan Agricultural University 28(2): 97-99

To increase oleate content in rape (B. napus (B. napus var. oleifera) XY15) and breed a cultivar high in oleate, the first strand of cDNA from the total RNA extracted from rape leaves was synthesized and used as a template for PCR, with oligonucleotide primers synthesized according to the FAD2 gene. PCR products were easily cloned into pGEM-T and sequenced.

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Accession: 003681581

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