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Cloning and sequencing of a gene encoding GOBP2 in the antenna of Spodoptera exigua


Cloning and sequencing of a gene encoding GOBP2 in the antenna of Spodoptera exigua



Agricultural Sciences in China 1(2): 200-206



ISSN/ISBN: 1671-2927

A pair of degenerate primers was designed, based on the comparison of five insects' GOBP2 gene sequences reported previously. A specific band (about 400bp in length) was amplified from cDNA of Spodoptera exigua antenna and another specific band (about 2kb in length) was amplified from genomic DNA. The two segments were cloned into T-easy vector, respectively.

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Accession: 003681583

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