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Cloning and sequencing of the S gene of H52 strain from avian infectious bronchitis virus


Cloning and sequencing of the S gene of H52 strain from avian infectious bronchitis virus



Journal of Zhejiang University Agriculture and Life Sciences 28(3): 303-306



Virion RNA from H52 strain of avian Infectious Bronchitis were extracted from the allantoic fluid of inoculated embryonated eggs and used as template for cDNA synthesis by reverse transcription with 3'-primer. Polymerase chain reaction was performed with two primers which span the S gene, and an expected product of 3.6 kb was obtained. The PCR product was ligated to pBlueScript SK vector, and its nucleotide sequence was determined by the dideoxy-mediated chain termination method.

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