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Cloning and sequencing the P125 gene of a Changchun isolate of bovine viral diarrhoea virus (BVDV)


Cloning and sequencing the P125 gene of a Changchun isolate of bovine viral diarrhoea virus (BVDV)



Chinese Journal of Veterinary Science and Technology 31(2): 3-6



Cytopathic BVDV NCD strain was propagated in the MDBK cell line and total RNA was extracted by a 1-step method of acid guanidium thiocyanate-phenol-chloroform extraction. A fragment of approximately 400 bp was amplified by reverse transcription-polymerase chain reaction (RT-PCR) with a pair of primers, W1/W2, which were synthesized using the published sequence of reference strain NADL.

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