Cloning of NA gene of avian influenza virus A/Goose/Guangdong/3/96 (H5N1) and construction of transfer vector of recombinant fowlpox virus

Qiao ChuanLing; Yu KangZhen; Deng GuoHua; Wang XiuRong; Meng QingWen; Tian GuoBin; Tang XiuYing

Chinese Journal of Veterinary Science 23(2): 111-114


Accession: 003681669

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The complementary DNA of NA gene of avian influenzavirus A/Goose/Guangdong/3/96(H5N1) isolate was amplified by reverse transcription-polymerase chain reaction (RT-PCR), then cloned into pUC18 and sequenced. The result of sequencing showed that 1410 bp of NA cDNA covered the complete open reading frame, encoding 468 amino acid residues. To construct the transfer vector, NA gene derived from recombinant plasmid pUC-NA was subcloned into EcoR I site of pSY538 and LacZ gene promoted by p11 was cloned into Sma I site of this plasmid. Both NA and LacZ gene were cloned into Not I site of FPV vector pSY681. Identified by restriction enzyme analysis and PCR, the transfer plasmid containing NA gene was constructed.