Cloning of a plastidic rye (Secale cereale) beta -glucosidase cDNA and its expression in Escherichia coli
Cloning of a plastidic rye (Secale cereale) beta -glucosidase cDNA and its expression in Escherichia coli
Nikus, J.; Esen, A.; Jonsson, L.M.V.
Physiologia Plantarum 118(3): 337-345
2003
ISSN/ISBN: 0031-9317
DOI: 10.1034/j.1399-3054.2003.00118.x
The cDNA for a beta -glucosidase (EC3.2.1.21) was isolated from rye (Secale cereale, cv Motto) and the sequence corresponding to the mature protein cloned into pET21a expression vector and used for transformation of Escherichia coli.