Cloning of bovine eukaryotic translation initiation factor 4E (eIF-4E) and its expression in the bovine mammary gland at different physiological stages
Cloning of bovine eukaryotic translation initiation factor 4E (eIF-4E) and its expression in the bovine mammary gland at different physiological stages
Long, E.; Capuco, A.V.; Zhao Xin
DNA Sequence 12(5/6): 319-329
2001
ISSN/ISBN: 1042-5179
DOI: 10.3109/10425170109084455
Bovine eIF-4E cDNA was cloned and its expression in the bovine mammary gland at distinct physiological stages was investigated. Bovine eIF-4E cDNA and protein sequences are highly homologous to those from other mammals. Using Northern blot analysis, we did not detect eIF-4E expression in the prepubertal bovine mammary gland, whereas a low level of eIF-4E mRNA was observed in the mammary tissues of heifers during the third oestrous cycle.