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Chapter 3,682

Cloning of cryptogein gene and construction of its plant expression vector

Jiang DongHua; Kong ShiHai; Guo ZeJian

Journal of Zhejiang University Agriculture and Life Sciences 28(6): 649-654

2002

Accession: 003681739
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The cryptogein genes was obtained by PCR from the genomic DNA of Phytophthora cryptogea and verified by DNA sequencing. A promoter of a rice phenylalanin ammonia-lyase (PAL) was selected to control the expression of the cryptogein gene. A chimaeric construct was designed in which the region of the cryptogein gene was fused to the signal sequence of the extracellular pathogenesis-related PR1b protein of tobacco. The final construct was cloned into a binary vector (CHF3).

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