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Cloning of the S1 gene of five Chinese isolates of avian infectious bronchitis virus and its identification


Cloning of the S1 gene of five Chinese isolates of avian infectious bronchitis virus and its identification



Chinese Journal of Veterinary Science 22(3): 222-225



Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to amplify the S1 gene fragments of the five Chinese isolates of infectious bronchitis virus (IBV) (HN2, HN4, JX1, SC2 and SC4) using the primers you1+ and you2-. The results indicated the size of the RT-PCR products was the same as expected (about 1.6 kb). The RT-PCR products were used as templates to amplify with you1+ and you2- with primers and were digested with the restriction enzyme Pst 1.

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