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Cloning, expression and characterization of the specific recombinant peptide of triose-phosphate isomerase of Schistosoma japonicum Chinese strain


Cloning, expression and characterization of the specific recombinant peptide of triose-phosphate isomerase of Schistosoma japonicum Chinese strain



Chinese Journal of Schistosomiasis Control 13(1): 9-13



To prepare the specific recombinant peptide of SjC-TPI by gene engineering technique. The DNA fragments of 15-66 aa and 129-163 aa regions of SjC-TPI which were the lowest homology corresponding to human TPI counterparts were amplified. Then the two gene fragments were connected together by gene ligation. The connected gene was sub-cloned into the expression vector pMAL-c2x, and subsequently expressed as a fusion protein with MBP in E. coli.

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