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Cloning, expression and identification of MPT-64 secreted proteins from Mycobacterium tuberculosis

Cloning, expression and identification of MPT-64 secreted proteins from Mycobacterium tuberculosis

Chinese Journal of Zoonoses 18(4): 1-4

A study was conducted to clone, identify and express the secreted protein MPT-64 from Mycobacterium tuberculosis and establish a foundation for the diagnosis of tuberculosis (TB), development of a TB vaccine and detection of immunity effectiveness. The gene encoding for protein MPT-64 was amplified from M. tuberculosis H37Rv genomic DNA by using PCR technique. The PCR product was cloned initially into the pGEX-4T-1 expression vector, then transformed into E. coli.

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