EurekaMag
+ Translate
+ Most Popular
Sympathetic and visceral nerve blocks
What should be done after bee or wasp stings?
Anti-drool cheiloplasty: clinical results in six dogs
The biology of human starvation: some new insights
Vitamin B12 deficiency as a worldwide problem
Effect of daily gelatin ingestion on human scalp hair
On the nature and role of vivotoxins in plant disease
History of the discovery of vitamins by N. I. Lunin
Preliminary tests with residual sprays against poultry lice
MDU.1, a new mutant chilli variety
A study on compatibility of some insecticides with a fungicide on small cardamom (Elettaria cardamomum Maton.)
The cranial nerves of Mystus seenghala (Sykes)
The effects of prefrontal lobotomy on aggressive behavior in dogs
Human monkeypox: disease pattern, incidence and attack rates in a rural area of northern Zaire
The identity of the lipstick mold of cultivated mushrooms agaricus bisporus
Therapy for acne with saccharomyces boulardii
Advantages and disadvantages of bordeaux mixture and of lime-sulphur used on apples in the growing season
The composition of pampas-grass (Cortaderia argentea.)
Productivity of Pekin x Khaki Campbell ducks
A rapid survey of katydids (Insecta: Orthoptera: Tettigoniidae) of the Foret Classee du Pic de Fon, Guinea
Cranial nerves of Labeo rohita (Ham.)
Evaluating fundamentals of care: The development of a unit-level quality measurement and improvement programme
Life-cycle of the free-living nematode Pontonema vulgare in the White Sea
Grey leaf blight - a new disease of cocoa
Effect of foliar spray of urea and zinc sulphate on yield and fruit quality of Kinnow - 'a mandarin hybrid'

Cold lability of lactate dehydrogenase isoenzymes and the effective preparation of reference material for clinical laboratory use


Cold lability of lactate dehydrogenase isoenzymes and the effective preparation of reference material for clinical laboratory use



Biotechnology and Applied Biochemistry 34(3): 167-171



ISSN/ISBN: 0885-4513

PMID: 11730484

DOI: 10.1042/ba20010002

To evaluate the storage of samples and enzyme reference materials, and to improve the commutability for inter-laboratory surveillance of activity values of lactate dehydrogenase (LD; EC 1.1.1.27) in clinical laboratory medicine and in animal veterinary medicine, we studied the electrophoretic patterns and cold lability of LD isoenzymes from tissue sources of some common vertebrate species and also from human serum sources. Among many isoenzymes from these sources, only rat LD fractions showed similar electrophoretic patterns to those of human sera, and rat LD-1 fraction was relatively cold-stable. Total LD and isoenzyme LD-1 activities in routine laboratory samples and quality-control sera were measured using eight kinds of commercially available LD assay kit, including lactate and pyruvate substrate systems. Coefficients of variation between these assay kits were markedly reduced when the activities were calculated using the partially purified rat LD-1 fraction as an enzyme reference material, compared with the activities calculated using the factor indicated in each assay kit. In the regression analysis, the intercept and slope were calculated for the regression equations obtained from 12 pairs of these assay kits. The values obtained from a small amount of human serum and control serum samples were within the 95% confidence regions of those from larger amounts of human serum samples by using the present rat LD-1 standard for measuring LD-1 activities with lactate substrate. It was evident that a cold-stable and homogeneous LD isoenzyme as an enzyme reference material might contribute to accurate measurement of activities in heterogeneous samples for inter-laboratory quality-assurance surveys in both human and animal clinical laboratory use.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 003682743

Download citation: RISBibTeXText

Related references

Immunochemical studies on lactate dehydrogenase: preparation and characterization of anti-M lactate dehydrogenase antiserum: development of an immunochemical assay for lactate dehydrogenase isoenzymes in human serum. Clinica Chimica Acta; International Journal of Clinical Chemistry 46(3): 205-215, 1973

Effects of cold acclimation on the activity levels of creatine kinase, lactate dehydrogenase and lactate dehydrogenase isoenzymes in various tissues of the rat. Cell Biology International 22(9-10): 701-707, 1998

Automation of Interpretive Clinical Laboratory Reports: Isoenzymes of Serum Lactate Dehydrogenase. Laboratory Medicine 10(10): 636-639, 1979

Cold lability found in a human serum lactate dehydrogenase. Journal of Clinical Chemistry and Clinical Biochemistry 19(8): 767, 1981

Studies on lactate dehydrogenase isoenzymes in human gingiva. II. The patterns of lactate dehydrogenase isoenzymes in periodontitis. Journal of Nihon University School of Dentistry 8(3): 123-129, 1966

Optimal conditions for assaying human lactate dehydrogenase by the lactate-to-pyruvate reaction: Arrhenium relationships for lactate dehydrogenase isoenzymes 1 and 5. Clinical Chemistry 23(7): 1289-1295, 1977

Comparative evaluation of the activity of lactate dehydrogenase, lactate dehydrogenase isoenzymes and malate dehydrogenase in ischemic heart disease. Terapevticheskii Arkhiv 41(11): 23-29, 1969

Lactate dehydrogenase and creatine phosphokinase isoenzymes in tissues of laboratory animals. Japanese Journal of Veterinary Research 38(1): 19-29, 1990

Studies on lactate dehydrogenase isoenzymes in human gingiva. I. Properties of lactate dehydrogenase isoenzymes in normal gingiva. Journal of Nihon University School of Dentistry 8(1): 27-32, 1966

Studies on lactate dehydrogenase isoenzymes in human gingiva. 3. The patterns of lactate dehydrogenase isoenzymes in fetal gingiva. Journal of Nihon University School of Dentistry 8(4): 186-190, 1966

Clinical significance of determination of the activity of lactate dehydrogenase, malate dehydrogenase and their isoenzymes in children with Botkin's disease. Voprosy Okhrany Materinstva i Detstva 17(9): 51-54, 1972

Reference values of lactate dehydrogenase (LDH), aspartate aminotransferase (GOT) and Aldolase isoenzymes in the plasma of children. Padiatrie und Grenzgebiete 17(5): 245-250, 1978

Study of the structural and functional features of lactate dehydrogenase isoenzymes, using antipeptide antibodies against porcine lactate dehydrogenase isoform M-4. Biokhimiya 57(5): 758-766, 1992

Study of the structure-activity features of lactate dehydrogenase isoenzymes using antipeptide antibodies to the M4-isoform of swine lactate dehydrogenase. Biokhimiia 57(5): 758-766, 1992

Serum lactate dehydrogenase isoenzymes 4 plus 5 is a better biomarker than total lactate dehydrogenase for refractory Mycoplasma pneumoniae pneumonia in children. Pediatrics and Neonatology 59(5): 501-506, 2018