EurekaMag
+ Translate
+ Most Popular
Investigations on the organic drift in North Swedish streams
Limitations of Using Microsoft Excel Version 2016 (MS Excel 2016) for Statistical Analysis for Medical Research
Phloem necrosis of coffee in Surinam
Anatomy of Mystus seenghala IV Nervous system
Integrated farming system for gardenland conditions of Coimbatore district - an over view
Tetrapleure (Tetrapleura tetraptera), an unknown African medicinal and spice plant
The origin and phylogenetic significance of the trochophoran larvae 2. evolutionary significance of the larvae of coelomate worms and mollusks
Comparison of rice bran and maize bran as feeds for growing and fattening pigs
Enterobacter amnigenus. An unusual human pathogen
Influence of Seriboost foliar application on leaf yield and leaf protein content in mulberry (Morus spp.), in relation to silkworm cocoon production
The identity of the lipstick mold of cultivated mushrooms agaricus bisporus
Advantages and disadvantages of bordeaux mixture and of lime-sulphur used on apples in the growing season
'Pan-sukh' disease of Rice in the Central Provinces
Geological age of the Ptilophyllum flora; a critical reassessment
Study of vitellogenesis in birds; physiological phases & role of folliculin in vitellogenesis
Evaluation of WCT coconut and Komadan coconut
Therapy for acne with saccharomyces boulardii
Evidence for Late Cretaceous N-S dextral shear in the west-central crystalline core, North Cascades, Washington
'Rajeshwari' - a high-yielding white seeded variety of sesame for Andhra Pradesh
Manufacture of Ricotta cheese from whey fortified with skim milk powder using different acidulants
Occurrence of Eutrichophilus mexicanus (Rudow, 1866) and Eutrichophilus lobatus (Ewing, 1936) (Phthiraptera: Trichodectidae) on Sphiggurus villosus (Cuvier, 1825) (Rodentia: Erethizontidae) in Rio de
Factors affecting fruitfulness in durian (Durio zibethinus Murr.). I. Flowering and pollination
Bronchial cuff pressure change caused by left-sided double-lumen endobronchial tube displacement
Some Biological Applications of Organometallic Compounds
The composition of pampas-grass (Cortaderia argentea.)

Combined analysis of supernatant-based feeding bioassays and PCR as a first-tier screening strategy for Vip -derived activities in Bacillus thuringiensis strains effective against tropical fall armyworm


Combined analysis of supernatant-based feeding bioassays and PCR as a first-tier screening strategy for Vip -derived activities in Bacillus thuringiensis strains effective against tropical fall armyworm



Journal of Applied Microbiology 93(2): 269-277



ISSN/ISBN: 1364-5072

PMID: 12147075

DOI: 10.1046/j.1365-2672.2002.01694.x

Aims: To assess whether feeding bioassays using culture-supernatant proteins could be combined with PCR into a first-tier screening strategy for Vip3A-like genes efficient against tropical Spodoptera frugiperda. Methods and Results: Out of 12 Bacillus thuringiensis strains studied, the total protein concentrated from the culture supernatant of only the strain HD125 yielded a significantly increased armyworm mortality and an intense band of the predicted size for VIP3A protein in SDS-PAGE. However, PCR and sequencing data indicated Vip-like genes are ubiquitous in tropical B. thuringiensis isolates. Interestingly, the HD125 strain was also the only one displaying a single-band amplification pattern and the highest sequence identity to the reported Vip3A(a) gene. Conclusions: Results suggest the insecticidal effectiveness of putative VIPs in B. thuringiensis isolates can be preliminarily estimated by the use of supernatant-derived total protein in feeding experiments, though only in a limited manner. Significance and Impact of the Study: A simple and cost-effective first-tier screening strategy for VIP-derived activities in B. thuringiensis collections can be developed by combining PCR and feeding bioassays. Moreover, the employed primers showed to be useful as a tool for strains differentiation at DNA level, and for characterization and isolation of Vip-like genes in tropical B. thuringiensis germplasm.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 003683487

Download citation: RISBibTeXText

Related references

Molecular characterization and distribution of Bacillus thuringiensis cry1 genes from Brazilian strains effective against the fall armyworm, Spodoptera frugiperda. Biological Control 53(3): 360-366, 2010

New isolate of Bacillus thuringiensis effective against the fall armyworm. Pesquisa Agropecuaria Brasileira 35(1): 221-227, 2000

Susceptibility of fall armyworm, Spodoptera frugiperda, to different strains of Bacillus thuringiensis. Revista Brasileira de Milho e Sorgo 3(1): 21-29, 2004

Characterization of Bacillus thuringiensis subsp. kurstaki strain S93 effective against the fall armyworm (Spodoptera frugiperda). Canadian Journal of Microbiology 45(6): 464-471, 1999

A genetically-modified Bacillus thuringiensis product effective for control of the fall armyworm (Lepidoptera: Noctuidae) on corn. Florida Entomologist 77(4): 437-440, 1994

The compatibility of Bacillus thuringiensis Cry protein-solubilizing buffers with the droplet feeding method in fall armyworm larvae. Journal of Invertebrate Pathology 166: 107233, 2019

PCR identification of CryI genes in Bacillus thuringiensis strains that are efficient against fall armyworm Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae). Anais da Sociedade Entomologica do Brasil 29(1): 147-153, 2000

A study on bioassays for toxicities of several highly effective strains of Bacillus thuringiensis to four species of lepidopterous larvae. Chinese Journal of Biological Control 11(1): 22-25, 1995

Growth variation among Bacillus thuringiensis strains can affect screening procedures for supernatant-secreted toxins against insect pests. Pest Management Science 67(9): 1184-1192, 2011

Requirement of simultaneous assessment of crystal- and supernatant-related entomotoxic activities of Bacillus thuringiensis strains for biocontrol-product development. Toxins 6(5): 1598-1614, 2014

Ecological factors affecting the pathogenecity of Bacillus thuringiensis var. thuringiensis to the European cons borer and fall armyworm. J. Invert. Path 8(3): 365-375, 1966

Susceptibility of conventional and transgenic cotton bolls expressing the Bacillus thuringiensis CryIA variant delta-endotoxin, to fall armyworm and beet armyworm injury. Journal of Agricultural Entomology 15(3): 163-171, 1998

Control of the African armyworm Spodoptera exempta Walker (Lepidoptera: Noctuidae) in Kenyan fields with highly effective strains of Bacillus thuringiensis Berliner. Biocontrol Science and Technology 1(2): 127-135, 1991

Screening of Bacillus thuringiensis strains effective against mosquitoes. Pesquisa Agropecuaria Brasileira 40(2): 103-106, 2005

Screening of Bacillus thuringiensis strains effective against mosquitoes. Pesquisa Agropecuaria Brasileira 40(2): 103-106, 2005