+ Translate
+ Most Popular
Advantages and disadvantages of bordeaux mixture and of lime-sulphur used on apples in the growing season
Observations on the Umaria marine bed
10 years of hearing conservation in the Royal Air Force
Chocolate crumb - dairy ingredient for milk chocolate
Effect of daily gelatin ingestion on human scalp hair
Comparison of rice bran and maize bran as feeds for growing and fattening pigs
The composition of pampas-grass (Cortaderia argentea.)
The Accraian Series:
The mechanism of the Liebermann-Burchard reaction of sterols and triterpenes and their esters
Cerebrovascular Doppler ultrasound studies (cv-Doppler)
Toria: PT-303 - first national variety
Hair growth promoting activity of tridax procumbens
Productivity of Pekin x Khaki Campbell ducks
A stable cytosolic expression of VH antibody fragment directed against PVY NIa protein in transgenic potato plant confers partial protection against the virus
Solar treatment of wheat loose smut
Swimmers itch in the Lake of Garda
Bactofugation and the Bactotherm process
The effects of prefrontal lobotomy on aggressive behavior in dogs
Visual rating scales for screening whorl-stage corn for resistance to fall armyworm
Breakdown of seamounts at the trench axis, viewed from gravity anomaly
Kooken; pennsylvania's toughest cave
Recovery of new dinosaur and other fossils from the Early Cretaceous Arundel Clay facies (Potomac Group) of central Maryland, U.S.A
Zubor horny (Bison bonasus) v prirodnych podmienkach Slovensku
The extended Widal test in the diagnosis of fevers due to Salmonella infection
Hair of the american mastodon indicates an adaptation to a semi aquatic habitat

Comparative analysis of the site-specific N-glycosylation of human lactoferrin produced in maize and tobacco plants

Comparative analysis of the site-specific N-glycosylation of human lactoferrin produced in maize and tobacco plants

European Journal of Biochemistry 270(15): 3235-3242

ISSN/ISBN: 0014-2956

PMID: 12869199

DOI: 10.1046/j.1432-1033.2003.03706.x

We have compared the site-by-site N-glycosylation status of human lactoferrin (Lf) produced in maize, a monocotyledon, and in tobacco, used as a model dicotyledon. Maize and tobacco plants were stably transformed and recombinant Lf was purified from both seeds and leaves. N-glycopeptides were generated by trypsin digestion of recombinant Lf and purified by reverse-phase HPLC. The N-glycosylation pattern of each site was determined by mass spectrometry. Our results indicated that the N-glycosylation patterns of recombinant Lf produced in maize and tobacco share common structural features. In particular, both N-glycosylation sites of each recombinant Lf are mainly substituted by typical plant paucimannose-type N-glycans, with beta1,2-xylose and alpha1,3-linked fucose at the proximal N-acetylglucosamine. However, tobacco Lf shows a significant amount of processed N-glycans with one or two beta1,2GlcNAc linked to the trimannose core, which are weakly expressed in maize Lf. Finally, no Lewisa epitope was observed on tobacco Lf.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 003684772

Download citation: RISBibTeXText

Related references

Site-specific O-glycosylation analysis of SARS-CoV-2 spike protein produced in insect and human cells. Biorxiv: the Preprint Server for Biology 2021, 2021

Site-specific glycosylation of human recombinant erythropoietin: analysis of glycopeptides or peptides at each glycosylation site by fast atom bombardment mass spectrometry. Biochemistry 27(23): 8618-8626, 1988

Heterogeneity in utilization of N-glycosylation sites Asn624 and Asn138 in human lactoferrin: a study with glycosylation-site mutants. Biochemical Journal 319: 117-122, 1996

Comprehensive characterization of the site-specific N-glycosylation of wild-type and recombinant human lactoferrin expressed in the milk of transgenic cloned cattle. Glycobiology 21(2): 206-224, 2011

Exploring Site-Specific N-Glycosylation of HEK293 and Plant-Produced Human IgA Isotypes. Journal of Proteome Research 16(7): 2560-2570, 2017

N-glycosylation potential of maize: the human lactoferrin used as a model. Glycoconjugate Journal 18(7): 519-527, 2001

Analysis of glycosylation site occupancy reveals a role for Ost3p and Ost6p in site-specific N-glycosylation efficiency. Molecular and Cellular Proteomics: Mcp 8(2): 357-364, 2009

Glycosylation site-specific analysis of HIV envelope proteins (JR-FL and CON-S) reveals major differences in glycosylation site occupancy, glycoform profiles, and antigenic epitopes' accessibility. Journal of Proteome Research 7(4): 1660-1674, 2008

Site-specific glycosylation of donkey milk lactoferrin investigated by high-resolution mass spectrometry. Amino Acids 48(12): 2799-2808, 2016

Site-specific N-glycosylation analysis of human immunoglobulin e. Journal of Proteome Research 13(2): 536-546, 2014

Site-specific glycosylation analysis of human apolipoprotein B100 using LC/ESI MS/MS. Glycobiology 15(5): 447-462, 2005

Site-Specific N- and O-Glycosylation Analysis of Human Plasma Fibronectin. Frontiers in Chemistry 9: 691217, 2021

Site-specific O-Glycosylation Analysis of Human Blood Plasma Proteins. Molecular and Cellular Proteomics: Mcp 15(2): 624-641, 2016

Comparative Site-Specific N-Glycosylation Analysis of Lactoperoxidase from Buffalo and Goat Milk Using RP-UHPLC-MS/MS Reveals a Distinct Glycan Pattern. Journal of Agricultural and Food Chemistry 66(43): 11492-11499, 2018

Site-specific N-glycosylation analysis of soluble Fcγ receptor IIIb in human serum. Scientific Reports 8(1): 2719, 2018