Ruminal degradations (in vitro) of DM and fibrous components in 9 substrates, besides gas production profile by anaerobic fungi which were compared in cattle and buffalo were influenced (P<0.01) by cell wall structural carbohydrates and varied between cattle and buffalo. Cattle fungi was better (P<0.01) than buffalo fungi in fermenting DM of substrates, which corroborated the pattern on ruminal gas production volume.