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Comparison of detection of Escherichia coli O157 between culture after acid treatment and Polymerase Chain Reaction after enrichment

Comparison of detection of Escherichia coli O157 between culture after acid treatment and Polymerase Chain Reaction after enrichment

Korean Journal of Laboratory Medicine 22(5): 331-335

ISSN/ISBN: 1598-6535

Background: The Polymerase Chain Reaction (PCR) for the Shiga toxin has been widely used for diagnosis of Shiga toxin-producing E. coli (STEC) infection including E. coli O157 (O157) instead of using a culture. However, bacteriological isolation must be followed for final diagnosis. Our study was aimed to compare the detection limit between the culture after the acid treatment and the PCR after enrichment.

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Accession: 003687098

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