+ Translate
+ Most Popular
Advantages and disadvantages of bordeaux mixture and of lime-sulphur used on apples in the growing season
Observations on the Umaria marine bed
10 years of hearing conservation in the Royal Air Force
Chocolate crumb - dairy ingredient for milk chocolate
Effect of daily gelatin ingestion on human scalp hair
Comparison of rice bran and maize bran as feeds for growing and fattening pigs
The composition of pampas-grass (Cortaderia argentea.)
The Accraian Series:
The mechanism of the Liebermann-Burchard reaction of sterols and triterpenes and their esters
Cerebrovascular Doppler ultrasound studies (cv-Doppler)
Toria: PT-303 - first national variety
Hair growth promoting activity of tridax procumbens
Productivity of Pekin x Khaki Campbell ducks
A stable cytosolic expression of VH antibody fragment directed against PVY NIa protein in transgenic potato plant confers partial protection against the virus
Solar treatment of wheat loose smut
Swimmers itch in the Lake of Garda
Bactofugation and the Bactotherm process
The effects of prefrontal lobotomy on aggressive behavior in dogs
Visual rating scales for screening whorl-stage corn for resistance to fall armyworm
Breakdown of seamounts at the trench axis, viewed from gravity anomaly
Kooken; pennsylvania's toughest cave
Recovery of new dinosaur and other fossils from the Early Cretaceous Arundel Clay facies (Potomac Group) of central Maryland, U.S.A
Zubor horny (Bison bonasus) v prirodnych podmienkach Slovensku
The extended Widal test in the diagnosis of fevers due to Salmonella infection
Hair of the american mastodon indicates an adaptation to a semi aquatic habitat

Construction and characterization of a BAC library for the molecular dissection of a single wild beet centromere and sugar beet (Beta vulgaris) genome analysis

Construction and characterization of a BAC library for the molecular dissection of a single wild beet centromere and sugar beet (Beta vulgaris) genome analysis

Genome 44(5): 846-855

ISSN/ISBN: 0831-2796

PMID: 11681609

DOI: 10.1139/g01-076

We have constructed a sugar beet bacterial artificial chromosome (BAC) library of the chromosome mutant PRO1. This Beta vulgaris mutant carries a single chromosome fragment of 6-9 Mbp that is derived from the wild beet Beta procumbens and is transmitted efficiently in meiosis and mitosis. The library consists of 50 304 clones, with an average insert size of 125 kb. Filter hybridizations revealed that approximately 3.1% of the clones contain mitochondrial or chloroplast DNA. Based on a haploid genome size of 758 Mbp, the library represents eight genome equivalents. Thus, there is a greater than 99.96% probability that any sequence of the PRO1 genome can be found in the library. Approximately 0.2% of the clones hybridized with centromeric sequences of the PRO1 minichromosome. Using the identified BAC clones in fluorescence in situ hybridization experiments with PRO1 and B. procumbens chromosome spreads, their wild-beet origin and centromeric localization were demonstrated. Comparative Southern hybridization of pulsed-field separated PRO1 DNA and BAC inserts indicate that the centromeric region of the minichromosome is represented by overlapping clones in the library. Therefore, the PRO1 BAC library provides a useful tool for the characterization of a single plant centromere and is a valuable resource for sugar beet genome analysis. Reprinted by permission of the publisher.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 003692091

Download citation: RISBibTeXText

Related references

Cytoplasmic male sterility in hybrids of sterile wild beet (Beta vulgaris ssp. maritima) and O-type fertile sugar beet (Beta vulgaris L.): molecular analysis of mitochondrial and nuclear genomes. Molecular Breeding 11(2): 137-148, 2003

Construction and characterization of a sugar beet (Beta vulgaris) fosmid library. Genome 51(11): 948-951, 2008

The effect of salt stress on lipid peroxidation and antioxidants in leaves of sugar beet Beta vulgaris L. and wild beet Beta maritima L. Plant Science (Oxford) 164(1): 77-84, 2003

The susceptibility of fodder beet and wild species of beta to an erwinia sp from sugar beet beta vulgaris. Plant Disease 66(8): 664-665, 1982

Comparison of chloroplast and mitochondrial DNA from five morphologically distinct Beta vulgaris cultivars: sugar beet, fodder beet, beet root, foliage beet, and Swiss chard. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 79(4): 440-442, 1990

Sugar beet beta vulgaris l monohy 149 palestriped flea beetle pfb systena blanda melsheimer sugar beet root maggot sbrm tetanops myopaeformis roeder sugar beet insect pests control 1989. Insecticide and Acaricide Tests : 232, 1991

Contribution of groundkeepers vs. weed beet to gene escape from sugar beet (Beta vulgaris spp.). Consequences for growing genetically-modified sugar beet - A modelling approach. Field Crops Research 135: 46-57, 2012

Molecular characterization and systemic induction of single-chain ribosome-inactivating proteins (RIPs) in sugar beet (Beta vulgaris) leaves. Journal of Experimental Botany 56(416): 1675-1684, 2005

What makes a transgenic plant an invasive alien? - Genetically modified sugar beet and their potential impact on populations of the wild beet Beta vulgaris subspec maritima arcang. Starfinger, U , Edwards, K , Kowarik, I , Williamson, M Plant invasions: Ecological mechanisms and human responses 235-243, 1998

Sugar beet beta vulgaris l beta seed kw 3265 sugar beet root aphid sra pemphigus spp sugar beet root aphid control in southern minnesota usa 1990. Insecticide and Acaricide Tests : 231-232, 1991

Occurrence of beet cryptic virus 1 and beet cryptic virus 2 in single plants of sugar beet and in other varieties of beta vulgaris l. Archiv fuer Phytopathologie und Pflanzenschutz 24(4): 273-281, 1988

Nuclear and cytoplasmic genetic diversity in weed beet and sugar beet accessions compared to wild relatives: new insights into the genetic relationships within the Beta vulgaris complex species. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 116(8): 1063-1077, 2008

Genome-wide identification, characterization, and expression patterns of the BZR transcription factor family in sugar beet (Beta vulgaris L.). Bmc Plant Biology 19(1): 191, 2019

Detection of Sugar Beet (Beta vulgaris) - Specific Gene from Honey Made by Sugar of Sugar Beet. Journal of Apiculture 33(3): 213-219, 2018

DsRNA-mediated resistance to Beet Necrotic Yellow Vein Virus infections in sugar beet (Beta vulgaris L. ssp. vulgaris). Molecular Breeding 18(4): 313-325, 2006