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Construction and expression of the recombinant plasmid pcDNA3.1/Ts87 of Trichinella spiralis


Construction and expression of the recombinant plasmid pcDNA3.1/Ts87 of Trichinella spiralis



Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 21(2): 93-95



ISSN/ISBN: 1000-7423

PMID: 12884619

Objective: To construct and express DNA plasmid of Trichinella spiralis. Methods: The BamHI and HindIII enzyme sites and KOZAK sequences were introduced at both ends of Ts87 gene by PCR. Ts87 gene was ligated into pcDNA3.1(+) vector with T4 ligase. The recombinant plasmid pcDNA3.1/Ts87 and plasmid pcDNA3.1 were transfected into an eukaryotic cell line COS7 through Lipofectamine, respectively.

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Accession: 003692119

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