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Construction and identification of a cDNA expression library from Clonorchis sinensis

Construction and identification of a cDNA expression library from Clonorchis sinensis

China Tropical Medicine 3(3): 282-284

In this study, a cDNA expression library for C. sinensis was constructed. Initially, total RNA from the helminth was extracted and cDNA was synthesized by SMART cDNA library protocol. The products were then digested and fractionated. The cDNA with sizes of 0.4-4 kb were recycled and ligated to an expression vector. An unamplified expression library was then produced. To test the quality of the obtained library, ten phages were randomly selected and amplified using specific primers.

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