+ Translate
+ Most Popular
Advantages and disadvantages of bordeaux mixture and of lime-sulphur used on apples in the growing season
Observations on the Umaria marine bed
10 years of hearing conservation in the Royal Air Force
Chocolate crumb - dairy ingredient for milk chocolate
Effect of daily gelatin ingestion on human scalp hair
Comparison of rice bran and maize bran as feeds for growing and fattening pigs
The composition of pampas-grass (Cortaderia argentea.)
The Accraian Series:
The mechanism of the Liebermann-Burchard reaction of sterols and triterpenes and their esters
Cerebrovascular Doppler ultrasound studies (cv-Doppler)
Toria: PT-303 - first national variety
Hair growth promoting activity of tridax procumbens
Productivity of Pekin x Khaki Campbell ducks
A stable cytosolic expression of VH antibody fragment directed against PVY NIa protein in transgenic potato plant confers partial protection against the virus
Solar treatment of wheat loose smut
Swimmers itch in the Lake of Garda
Bactofugation and the Bactotherm process
The effects of prefrontal lobotomy on aggressive behavior in dogs
Visual rating scales for screening whorl-stage corn for resistance to fall armyworm
Breakdown of seamounts at the trench axis, viewed from gravity anomaly
Kooken; pennsylvania's toughest cave
Recovery of new dinosaur and other fossils from the Early Cretaceous Arundel Clay facies (Potomac Group) of central Maryland, U.S.A
Zubor horny (Bison bonasus) v prirodnych podmienkach Slovensku
The extended Widal test in the diagnosis of fevers due to Salmonella infection
Hair of the american mastodon indicates an adaptation to a semi aquatic habitat

Construction of a Sinorhizobium meliloti strain carrying a stable and non-transmissible chromosomal single copy of the green fluorescent protein GFP-P64L/S65T

Construction of a Sinorhizobium meliloti strain carrying a stable and non-transmissible chromosomal single copy of the green fluorescent protein GFP-P64L/S65T

FEMS Microbiology Letters 214(2): 165-170

ISSN/ISBN: 0378-1097

PMID: 12351225

DOI: 10.1111/j.1574-6968.2002.tb11341.x

A single copy of the green fluorescent protein (GFP)-encoding gene gfp-P64L/S65T under the control of the constitutive nptII promoter was introduced in a neutral region of the Sinorhizobium meliloti chromosome, between the genes recA and alaS. Within the same chromosomal region downstream of gfp-P64L/S65T a tetracycline (Tc) resistant cassette was also inserted. Both markers were very stable during at least 40 bacterial generations without any selective pressure. Similarly, the gfp-Tc cassette was stable and functional in all rhizobia that were recovered from alfalfa nodules. The GFP-associated fluorescence derived from the (single copy) chromosomal gfp-P64L/S65T allowed detection of rhizobia during the colonisation of the root, infection thread formation, and nodule development. The gfp-Tc rhizobia showed indistinguishable phenotypes for nodulation, competitiveness, and nitrogen-fixation from the parental strain. The labelling system described here can be used for the stable fluorescent tagging of S. meliloti strains allowing their detection in biologically complex soil environments.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 003692186

Download citation: RISBibTeXText

Related references

Identification of a transmissible plasmid from an Argentine Sinorhizobium meliloti strain which can be mobilised by conjugative helper functions of the European strain S. meliloti GR4. FEMS Microbiology Letters 225(1): 15-21, 2003

Construction and characterization of a fluorescent sendai virus carrying the gene for envelope fusion protein fused with enhanced green fluorescent protein. Bioscience Biotechnology and Biochemistry 74(11): 2293-2298, 2010

A Family of Single Copy repABC-Type Shuttle Vectors Stably Maintained in the Alpha-Proteobacterium Sinorhizobium meliloti. Acs Synthetic Biology 6(6): 968-984, 2017

Construction and application of retroviral vector carrying green fluorescent protein. Zhonghua Yi Xue Yi Chuan Xue Za Zhi 15(4): 232-234, 1998

Construction and environmental release of a Sinorhizobium meliloti strain genetically modified to be more competitive for alfalfa nodulation. Applied and Environmental Microbiology 67(9): 3860-3865, 2001

Identification of chromosomal alpha-proteobacterial small RNAs by comparative genome analysis and detection in Sinorhizobium meliloti strain 1021. Bmc Genomics 8: 467, 2007

The chromosomal replication initiation protein DnaA, and the identification of the chromosomal origin of replication from Sinorhizobium meliloti. Finan, Turlough M , Reprint Author, O'Brian, Mark R, Layzell, David B, Vessey, J Kevin, Newton, William Nitrogen fixation: Global perspectives: 390, 2002

Construction of self-transmissible green fluorescent protein-based biosensor plasmids and their use for identification of N-acyl homoserine-producing bacteria in lake sediments. Applied and Environmental Microbiology 76(18): 6119-6127, 2010

Sinorhizobium fredii and Sinorhizobium meliloti produce structurally conserved lipopolysaccharides and strain-specific K antigens. Applied and Environmental Microbiology 64(12): 4930-4938, 1998

Construction of transgenic mice carrying enhanced green fluorescent protein gene by seminiferous tubule microinjection. Di 1 Jun Yi da Xue Xue Bao 22(3): 250-253, 2002

Construction of the enhanced green fluorescent protein eukaryotic expression vector carrying CagA of Helicobacter pylori. Zhonghua Yi Xue Za Zhi 87(8): 570-572, 2007

Construction and characterization of a recombinant ovine lentivirus carrying the optimized green fluorescent protein gene at the dUTPase locus. Archives of Virology 148(8): 1485-1506, 2003

Stable low molecular weight RNA profiling showed variations within Sinorhizobium meliloti and Sinorhizobium medicae nodulating different legumes from the alfalfa cross-inoculation group. Fems Microbiology Letters 282(2): 273-281, 2008

Growth and nodulation competitiveness of Sinorhizobium meliloti L1 is less than that of its isogenic strain L33 but comparable to that of two S meliloti wild-type isolates. Applied Microbiology and Biotechnology 47(5): 525-529, 1997

Polyphasic taxonomy of rhizobia: Emendation of the genus Sinorhizobium and description of Sinorhizobium meliloti comb. nov., Sinorhizobium saheli sp. nov., and Sinorhizobium teranga sp. nov. International Journal of Systematic Bacteriology 44(4): 715-733, 1994