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Construction of a universal transfer vector deleting gG gene of pseudorabies virus

Construction of a universal transfer vector deleting gG gene of pseudorabies virus

Journal of Huazhong Agricultural University 20(4): 306-309

To construct a universal transfer vector (UTV) deleting Gg gene of pseudorabies virus (PRV), the plasmid pBSA containing the Sph 15 kb fragment of genomic DNA of PRV Ea strain was digested with Kpn 1 and Sph 1.

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