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Construction of an expression vector PGEX-5X-1-ospC carrying the outer surface protein C gene from Borrelia garinii PD19 strain and sequence determination


Construction of an expression vector PGEX-5X-1-ospC carrying the outer surface protein C gene from Borrelia garinii PD19 strain and sequence determination



Chinese Journal of Zoonoses 19(4): 19-21



An expression vector was constructed carrying the Borrelia garinii major outer surface protein C (OspC) gene, which was cloned and expressed for the diagnosis of Lyme disease. The OspC gene was amplified from the genome of B. garinii PD91 strain by polymerase chain reaction (PCR) and recombined with plasmid PGEX-5X-1. The recombinant plasmid, PGEX-5X-1-OspC was identified with PCR, restriction endonuclease analysis and sequencing.

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