Construction of binary expressing vector containing GNA gene and alpha -PAP gene and transformation of the vector into Nicotiana tabacum
Chai HongMei; Zhang ShaoSong; Wan Meng; Zhao YongChang; Huang XingQi
Southwest China Journal of Agricultural Sciences 15(4): 35-37
2002
Accession: 003692254
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A plant expression vector with resistance to both virus and aphid was constructed by inserting the Galanthus nivalis agglutinin gene (GNA) and the pokeweed antiviral protein gene (pHI121/ alpha -PAP). GNA and alpha -PAP were transferred into tobacco using Agrobacterium tumefaciens-mediated transformation system and under kanamycin selection pressure.
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