Construction of double haploid (DH) and recombinant inbred line (RIL) population of indica-japonica hybrid and their differential in indica and japonica property

Cheng ShiHua; Mao ChuanZao; Zhan XiaoDeng; Si HuaMin; Sun ZongXiu

Chinese Journal of Rice Science 15(4): 257-260

2001


ISSN/ISBN: 1001-7216
Accession: 003692265

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Abstract
A double haploid (DH) population with 132 lines, and recombinant inbred lines (RIL) with 152 lines, were constructed by one-step anther culture and continuous self-pollination of the hybrid between rice cultivars 02428 (japonica) and Teqing (indica), respectively. The differentiation of indica and japonica in the two populations was detected by morphological index and RFLP method. All morphological traits of DH and RIL populations were continuously segregating and exhibited a normal distribution. The segregation and recombination of indica and japonica genes in the DH population were basically random, and the overall frequency of alleles did not deviate from the ratio 1 : 1. The RIL population significantly tended to the indica parent.