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Construction of recombinant baculovirus expressing S1 glycoprotein of infectious bronchitis virus isolate JS/95/03


Construction of recombinant baculovirus expressing S1 glycoprotein of infectious bronchitis virus isolate JS/95/03



Chinese Journal of Virology 19(1): 86-90



ISSN/ISBN: 1000-8721

The recombinant baculovirus expressing S1 glycoprotein of nephropathogenic strain JS/95/03 of infectious bronchitis virus was generated by using the Bac-to-Bac baculovirus expression system. The Bam H I-Sal I fragment containing S1 gene from the recombinant plasmid pMDJS9503S1 was purified and cloned in frame into the baculovirus transposing vector pFASTBAC HTa under the polyhedrin gene promoter.

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