Construction of recombinant baculovirus expressing S1 glycoprotein of infectious bronchitis virus isolate JS/95/03
Construction of recombinant baculovirus expressing S1 glycoprotein of infectious bronchitis virus isolate JS/95/03
Dai YaBin; Chen DeSheng; Ding Chan; Pan JieYan; Liu XingYou; L.Y.nHua; Liu Mei; Chen PuYan; Cai BaoXiang
Chinese Journal of Virology 19(1): 86-90
2003
ISSN/ISBN: 1000-8721
The recombinant baculovirus expressing S1 glycoprotein of nephropathogenic strain JS/95/03 of infectious bronchitis virus was generated by using the Bac-to-Bac baculovirus expression system. The Bam H I-Sal I fragment containing S1 gene from the recombinant plasmid pMDJS9503S1 was purified and cloned in frame into the baculovirus transposing vector pFASTBAC HTa under the polyhedrin gene promoter.