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Construction of sequencing recombinant plasmid and eukaryotic expression recombinant plasmid of CTP (phosphocholine cytidylytransferase) from Plasmodium falciparum (FCC1/HN)


Construction of sequencing recombinant plasmid and eukaryotic expression recombinant plasmid of CTP (phosphocholine cytidylytransferase) from Plasmodium falciparum (FCC1/HN)



Chinese Journal of Zoonoses 18(3): 22-24



A study was conducted to construct a eukaryotic expression vector of the CTP gene and study its function. The published CTP gene sequence was used to design and synthesize a pair of primers. The CTP gene was amplified by PCR and cloned into vector pUC19. After digestion with HindII and BamHI, the CTP gene was ligated to the eukaryotic expression vector pcDNA3. The sequence of the CTP gene was proven correct.

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