Section 4
Chapter 3,695

Conversion of pyridylamino sugar chains to corresponding reducing sugar chains

Takahashi, C.; Nakakita, S-ichi.; Hase, S.

Journal of Biochemistry 134(1): 51-55


ISSN/ISBN: 0021-924X
PMID: 12944370
DOI: 10.1093/jb/mvg112
Accession: 003694704

Download citation:  

Full Text Article emailed within 0-6 h
Payments are secure & encrypted
Powered by Stripe
Powered by PayPal

Pyridylamino sugar chains were converted to the corresponding reducing sugar chains by first converting them to 1-amino-1-deoxy derivatives using the method previously reported [S. Hase, J. Biochem. 112, 266-268 (1992)] and then converting the products to the corresponding reducing sugar chains using the Sommlet reaction. The reaction conditions were optimized so as to obtain the maximal product yield using 1-amino-1-deoxylactose and 1-amino-1-deoxy-N-acetylglucosamine. When the established procedure was successively applied to pyridylamino high-mannose and complex-type sugar chains, the corresponding reducing sugar chains were obtained in yields of 30%.