Conversion of pyridylamino sugar chains to corresponding reducing sugar chains
Takahashi, C.; Nakakita, S-ichi.; Hase, S.
Journal of Biochemistry 134(1): 51-55
ISSN/ISBN: 0021-924X PMID: 12944370 DOI: 10.1093/jb/mvg112
Pyridylamino sugar chains were converted to the corresponding reducing sugar chains by first converting them to 1-amino-1-deoxy derivatives using the method previously reported [S. Hase, J. Biochem. 112, 266-268 (1992)] and then converting the products to the corresponding reducing sugar chains using the Sommlet reaction. The reaction conditions were optimized so as to obtain the maximal product yield using 1-amino-1-deoxylactose and 1-amino-1-deoxy-N-acetylglucosamine. When the established procedure was successively applied to pyridylamino high-mannose and complex-type sugar chains, the corresponding reducing sugar chains were obtained in yields of 30%.