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Cross-species amplification of the Hordeum chilense genome using barley Sequence-Tagged-Sites (STSs)


Cross-species amplification of the Hordeum chilense genome using barley Sequence-Tagged-Sites (STSs)



Hereditas Lund 135(2/3): 243-246



DOI: 10.1111/j.1601-5223.2001.00243.x

A selection of 51 barley Sequence-Tagged Sites (STSs) were studied for their utility in Hordeum chilense. They included four primer sets from wheat origin and six primer sets from oat origin. Forty-four primer pairs amplified H. chilense products consistently. Five primer pairs were suitable for studying the introgression of H. chilense in wheat because they amplified H. chilense products of distinct size. Six of the STSs showed polymorphism between different H. chilense accessions.

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Accession: 003697384

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