Cryopreservation of grapevine (Vitis vinifera L.) embryogenic cell suspensions by encapsulation-dehydration and subsequent plant regeneration
Cryopreservation of grapevine (Vitis vinifera L.) embryogenic cell suspensions by encapsulation-dehydration and subsequent plant regeneration
Wang QiaoChun; Gafny, R.; Sahar, N.; Ilan Sela; Munir Mawassi; Tanne, E.; Avihai Perl
Plant Science 162(4): 551-558
2002
ISSN/ISBN: 0168-9452
DOI: 10.1016/s0168-9452(01)00594-5
Embryogenic cell suspensions of grapevine (Vitis vinifera L.) were successfully cryopreserved by encapsulation-dehydration and subsequently regenerated into plants. Embryogenic cell suspensions were first-step precultured for 2 days in MGN liquid medium supplemented with increasing sucrose concentrations of 0.25, 0.5, 0.75 and 1 M. Precultured cells were then encapsulated and second-step precultured in MGN liquid medium containing 1 M sucrose for several days.