EurekaMag
+ Translate
+ Most Popular
Sympathetic and visceral nerve blocks
What should be done after bee or wasp stings?
Anti-drool cheiloplasty: clinical results in six dogs
The biology of human starvation: some new insights
Vitamin B12 deficiency as a worldwide problem
Effect of daily gelatin ingestion on human scalp hair
On the nature and role of vivotoxins in plant disease
History of the discovery of vitamins by N. I. Lunin
Preliminary tests with residual sprays against poultry lice
MDU.1, a new mutant chilli variety
A study on compatibility of some insecticides with a fungicide on small cardamom (Elettaria cardamomum Maton.)
The cranial nerves of Mystus seenghala (Sykes)
The effects of prefrontal lobotomy on aggressive behavior in dogs
Human monkeypox: disease pattern, incidence and attack rates in a rural area of northern Zaire
The identity of the lipstick mold of cultivated mushrooms agaricus bisporus
Therapy for acne with saccharomyces boulardii
Advantages and disadvantages of bordeaux mixture and of lime-sulphur used on apples in the growing season
The composition of pampas-grass (Cortaderia argentea.)
Productivity of Pekin x Khaki Campbell ducks
A rapid survey of katydids (Insecta: Orthoptera: Tettigoniidae) of the Foret Classee du Pic de Fon, Guinea
Cranial nerves of Labeo rohita (Ham.)
Evaluating fundamentals of care: The development of a unit-level quality measurement and improvement programme
Life-cycle of the free-living nematode Pontonema vulgare in the White Sea
Grey leaf blight - a new disease of cocoa
Effect of foliar spray of urea and zinc sulphate on yield and fruit quality of Kinnow - 'a mandarin hybrid'

Cryopreservation of oocytes from pre-antral follicles


Cryopreservation of oocytes from pre-antral follicles



Human Reproduction Update 9(2): 119-129



ISSN/ISBN: 1355-4786

PMID: 12751774

DOI: 10.1093/humupd/dmg014

Cryobiology is a very important tool in reproductive biology. Research in this area focuses on the possibility of restoring fertility in women with reproductive problems or after cancer treatments. Another goal is to establish a genetic resource bank for endangered or commercially important animal species. Cryopreservation of oocytes from pre-antral follicles has been studied during the past decade. Procedures can be divided between the cryopreservation of either ovarian tissue or isolated follicles. Most studies describe a slow freezing/rapid thawing protocol to cryopreserve ovarian fragments. Histology shows that the follicles maintain their morphological integrity, and transplantation of ovarian tissue demonstrates that the follicles can restart their growth and eventually ovulate. Some research groups have obtained offspring using this procedure in mice and sheep. With regard to the cryopreservation of isolated follicles, the few studies reported in this area used the same freezing protocol, and some of them described follicular growth using in-vitro culture. The best result was obtained in mice, with animal birth after follicular cryopreservation and culture. However, additional studies are necessary for a better understanding of the events during follicular cryopreservation and to establish a standard protocol for ovarian transplantation or follicle culture.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 003697596

Download citation: RISBibTeXText

Related references

Co-culture of buffalo (Bubalus bubalis) preantral follicles with antral follicles: a comparative study of developmental competence of oocytes derived from in vivo developed and in vitro cultured antral follicles. Zygote 21(3): 286-294, 2013

Ovulation trigger and retrieval of GV and MI oocytes from antral follicles developing from primary and preantral follicles in culture: are we getting closer to obtaining mature oocytes from in vitro grown follicles in human?. Reproductive Biomedicine Online 28: S2-S3, 2014

Vitrification of in vitro matured oocytes collected from antral follicles at the time of ovarian tissue cryopreservation. Reproductive Biology and Endocrinology: Rb&e 9: 150, 2011

Embryo transfer following in vitro maturation and cryopreservation of oocytes recovered from antral follicles during conservative surgery for ovarian cancer. Journal of Assisted Reproduction and Genetics 29(8): 779-781, 2012

Effect of cryopreservation on the ability of granulosa cells to support in vitro development of oocytes derived from porcine early antral follicles. Theriogenology 143: 50-56, 2020

In situ hybridization and biochemical evidence for expression of cholinesterase genes in oocytes from human primordial pre antral and antral follicles. Human Reproduction 2(Suppl. 1): 50, 1987

The relationship between antral follicle count in a bovine ovary and developmental competence of in vitro-grown oocytes derived from early antral follicles. Biomedical Research 37(1): 63-71, 2016

A comparison of in vitro culture of oocytes with surrounding cumulus and granulosa cells from bovine early antral follicles with same-size whole, intact follicles. Theriogenology 53(1): 469, 2000

Effects of reaggregated granulosa cells and oocytes derived from early antral follicles on the properties of oocytes grown in vitro. Journal of Reproduction and Development 61(3): 191-197, 2015

In vitro culture of secondary follicles and prematuration of cumulus-oocyte complexes from antral follicles increase the levels of maturation-related transcripts in bovine oocytes. References 86(12): 1874-1886, 2019

Stage-dependent effects of oocytes and growth differentiation factor 9 on mouse granulosa cell development: advance programming and subsequent control of the transition from preantral secondary follicles to early antral tertiary follicles. Biology of Reproduction 70(5): 1253-1262, 2004

Ultrastructure changes in the oocytes of caprine antral follicles. Indian Journal of Animal Sciences 68(4): 332-336, 1998

Cryopreservation and development of antral follicles in cat ovarian tissue after transplantation into NOD SCID mice. Theriogenology 57(1): 362, 2002

Antral follicles confer developmental competence on oocytes. Zygote 4(4): 289-293, 1996

The determination of atretic changes in oocytes from antral follicles of cows. Sostoyanie i perspektivy razvitiya biotekhnologii v zhivotnovodstve, Khar' kov, 21-22 sentyabrya 1988: 78-79, 1988