Crystallization and preliminary crystallographic studies of juvenile hormone-binding protein from Galleria mellonella haemolymph
Crystallization and preliminary crystallographic studies of juvenile hormone-binding protein from Galleria mellonella haemolymph
Kołodziejczyk, R.; Kochman, M.; Bujacz, G.; Dobryszycki, P.; Ozyhar, A.; Jaskolski, M.
Acta Crystallographica. Section D Biological Crystallography 59(Pt 3): 519-521
2003
ISSN/ISBN: 0907-4449
PMID: 12595713
DOI: 10.1107/s0907444902022904
Juvenile hormone-binding protein (JHBP) isolated from Galleria mellonella haemolymph has been crystallized using the hanging-drop method in two polymorphic forms. The best diffracting crystals (2.7 A) are trigonal, space group P3(1)21 (or P3(2)21), with unit-cell parameters a = 110.4, c = 93.9 A. X-ray diffraction data have been collected for the native crystals using synchrotron radiation and cryogenic conditions (100 K).