Cultivar discrimination among wakegi onion (Allium x wakegi Araki) by fluorescent AFLP technique
Araki, N.; Furuta, T.; Ohara, T.; Yamauchi, N.; Shigyo, M.
Journal of the Japanese Society for Horticultural Science 72(3): 230-235
Nine virus-free clones (Hiroshima 1 go-Hiroshima 9 go) of wakegi onion (A. wakegi) were examined by fluorescent amplified fragment length polymorphism (AFLP) technique using 16 primer combinations. Twelve out of the 16 primer combinations amplified a total of 678 DNA fragments (peaks) in nine wakegi onion clones. Eight primer combinations produced 11 polymorphic markers available for discriminating the nine clones from each other, except between Hiroshima 1 go ('Shimonoseki') and Hiroshima 2 go ('Kanshirazu'). The AFLP data revealed that 26.3% and 23.5% of the 678 DNA fragments were derived from Japanese bunching onion (Allium fistulosum) and shallot, respectively.