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Cultivar identification in upland cotton using RAPD markers


Cultivar identification in upland cotton using RAPD markers



Australian Journal of Agricultural Research 53(6): 637-642



ISSN/ISBN: 0004-9409

DOI: 10.1071/ar01129

Using the random amplified polymorphic DNA (RAPD) method, the identification and the genetic description of 28 upland cotton (Gossypium hirsutum L.) cultivars currently cultivated in Greece was attempted. Based on the results of a preliminary experiment using 50 ten-base arbitrary primers, 24 were selected for the main experiment. DNA bands totaling 181 were observed, 118 (65.2%) of which were polymorphic. On the average, 7.5 DNA bands were amplified per primer, 4.9 of which were polymorphic.

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