Cytogenetic analysis of an asymmetric potato hybrid
Cytogenetic analysis of an asymmetric potato hybrid
Srebniak, Młgorzata.; Rasmussen, O.; Małuszyńska, J.
Journal of Applied Genetics 43(1): 19-31
2002
ISSN/ISBN: 1234-1983
PMID: 12084967
An asymmetric potato hybrid (FAO1-1A) and its parental lines (Solanum wild-type V8 and S. tuberosum FOLVA) were cytogenetically examined. DAPI (4'-6-diamidino-2-phenylindole) staining was used to count chromosomes in all analysed lines and revealed the presence of minichromosomes in the hybrid genome. Fluorescent in situ hybridization (FISH) with rDNA sequence as a probe helped to determine the ploidy level of analysed lines and revealed that none of the minichromosomes contains rDNA repeats.