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Cytoplasmic male sterility in hybrids of sterile wild beet (Beta vulgaris ssp. maritima) and O-type fertile sugar beet (Beta vulgaris L.): molecular analysis of mitochondrial and nuclear genomes



Cytoplasmic male sterility in hybrids of sterile wild beet (Beta vulgaris ssp. maritima) and O-type fertile sugar beet (Beta vulgaris L.): molecular analysis of mitochondrial and nuclear genomes



Molecular Breeding 11(2): 137-148



The organization of the mitochondrial genome of B3, B4 and B5 generations of hybrids created by backcrossing sterile wild beet Beta maritima with a fertile O-type sugar beet line was studied using restriction fragment length polymorphism (RFLP) analysis. Random amplified polymorphic DNA (RAPD) analysis was used to study restoration of the fertile (O-type) sugar beet genotype in hybrids after multiple backcrossings. Restriction of mtDNAs from the cytoplasm of B. maritima and hybrids revealed BamHI, EcoRI and XhoI restriction patterns different from those for sterile and fertile sugar beet lines. The most conspicuous feature of our accession of sterile wild beet mtDNA was the absence of the 10.7-kb EcoRI fragment detected in the cytoplasm of S-type sterile B. maritima and sugar beet. The hybridization of digested mtDNAs with coxII, atpA and atp6 homologous probes revealed alterations within these gene loci that distinguished wild beet and hybrids from sugar beets. Characteristic hybridization profiles for the wild beet and B3, B4 and B5 hybrids were observed for all probes regardless of the restrictase used to digest mtDNA. Notable changes in atpA and atp6 genes resulted when probes that comprised the 5' flanking sequences of these genes and a small part of the coding sequences were used. RFLP analysis of the sterile B. maritima mitochondrial genome further supported the unique character of this source of wild beet sterility. The genotypic differences between hybrids and parental accessions were determined by scoring PCR-RAPD reaction products for nine selected primers. The diversity of the B. maritima genotype resulted in a lower genetic similarity index in comparison with hybrids, sterile and fertile lines of sugar beet. The dendrogram obtained after cluster analysis distinguished hybrids as a group that differed from wild beet and the maintainer sugar beet line used for backcrossing. These results may indicate incomplete restoration of the fertile sugar beet genotype in hybrids.

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Accession: 003699849

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DOI: 10.1023/a:1022450430434


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