Deficiency of glutathione peroxidase-1 sensitizes hyperhomocysteinemic mice to endothelial dysfunction
Dayal, S.; Brown, K.L.; Weydert, C.J.; Oberley, L.W.; Arning, E.; Bottiglieri, T.; Faraci, F.M.; Lentz, S.R.
Arteriosclerosis Thrombosis and Vascular Biology 22(12): 1996-2002
ISSN/ISBN: 1079-5642 PMID: 12482825 DOI: 10.1161/01.atv.0000041629.92741.dc
Objective - We tested the hypothesis that deficiency of cellular glutathione peroxidase (GPx-1) enhances susceptibility to endothelial dysfunction in mice with moderate hyperhomocysteinemia. Methods and Results - Mice that were wild type (Gpx1+/+), heterozygous (Gpx1+/-), or homozygous (Gpx1-/-) for the mutated Gpx1 allele were fed a control diet or a high-methionine diet for 17 weeks. Plasma total homocysteine was elevated in mice on the high-methionine diet compared with mice on the control diet (23+or-3 versus 6+or-0.3 micro mol/L, respectively; P<0.001) and was not influenced by Gpx1 genotype.