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Detection and differentiation of European and American genotypes of porcine reproductive and respiratory syndrome virus (PRRSV) by modified one tube reverse transcriptase nested polymerase chain reaction (RT-PCR)


Detection and differentiation of European and American genotypes of porcine reproductive and respiratory syndrome virus (PRRSV) by modified one tube reverse transcriptase nested polymerase chain reaction (RT-PCR)



Veterinarija ir Zootechnika 21: 29-36



A single tube nested reverse transcription-polymerase chain reaction (RT-PCR) was developed for rapid detection and identification of PRRSV. The aim of the study was to apply the simplified procedure of reverse transcription and polymerase chain reaction (RT nested PCR) to diagnose European-type and American-type of PRRSV. Total RNA was extracted from virus-containing cell culture supernatant and porcine serum samples.

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