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Detection of Cryptococcus neoformans DNA in tissue samples by nested and real-time PCR assays

Detection of Cryptococcus neoformans DNA in tissue samples by nested and real-time PCR assays

Clinical and Diagnostic Laboratory Immunology 9(2): 461-469

ISSN/ISBN: 1071-412X

PMID: 11874894

DOI: 10.1128/cdli.9.2.461-469.2002

Two PCR protocols targeting the 18S rRNA gene of Cryptococcus neoformans were established, compared, and evaluated in murine cryptococcal meningitis. One protocol was designed as a nested PCR to be performed in conventional block thermal cyclers. The other protocol was designed as a quantitative single-round PCR adapted to LightCycler technology. One hundred brain homogenates and dilutions originating from 20 ICR mice treated with different azoles were examined.

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Accession: 003704365

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