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Detection of Leptospira spp. from pure cultures and from experimentally contaminated bovine semen by polymerase chain reaction


Detection of Leptospira spp. from pure cultures and from experimentally contaminated bovine semen by polymerase chain reaction



Brazilian Journal of Veterinary Research and Animal Science 36(1/3): 15-18



ISSN/ISBN: 1413-9596

Due to the high importance of the venereal transmission of bovine leptospirosis, this study tested the ability of PCR to detect Leptospira interrogans serovar hardjo DNA in experimentally contaminated bovine semen. Employing primers directed to the 16S rRNA gene, 10 bacteria/ml of semen could be detected by PCR.

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Accession: 003704460

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