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Detection of antibiotics by using minimum detectable limit concentrations (MDCs) in the swab test on premise (stop) assay and thin layer chromatography - bioautography (TLCB) of agar blocks


Detection of antibiotics by using minimum detectable limit concentrations (MDCs) in the swab test on premise (stop) assay and thin layer chromatography - bioautography (TLCB) of agar blocks



Journal of the Faculty of Veterinary Medicine, University of Tehran 56(1): Pe20-Pe24



Nineteen antibiotics were evaluated using a thin layer chromatographic-bioautographic method (TLCB); also, a parallel evaluation was done of the standard curves of the antibiotics. The sensitivity of the method and the amount of drugs were determined by measuring the amount of minimum detectable limit concentrations (MDCs, micro g/ml), zone size (Z) and the rate of flow for each antibiotic (RF). Bacillus subtilis ATCC 6633 was used as the test organism.

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