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Detection of cucumber mosaic virus, lily symptomless virus and lily mottle virus in Lilium species by RT-PCR technique


Detection of cucumber mosaic virus, lily symptomless virus and lily mottle virus in Lilium species by RT-PCR technique



Scientia Horticulturae 97(1): 57-63, 3 January



ISSN/ISBN: 0304-4238

DOI: 10.1016/s0304-4238(02)00125-5

Cucumber mosaic virus (CMV), lily symptomless virus (LSV) and lily mottle virus (LMoV) were detected in three lily cultivars by reverse transcription-polymerase chain reaction (RT-PCR). Results were compared with those obtained in an enzyme-linked immunosorbent assay (ELISA). The amplified DNA fragments with expected size and specific bands for each virus were obtained by RT-PCR using degenerate primers. In 18 out of 22 plants tested at least one virus was detected with RT-PCR in the 18 (81.8%) plants compared to 4 (18.2%) plants with ELISA. The present study showed that the RT-PCR is a more sensitive method than ELISA to determine the presence of viruses in Lilium plants of various species.

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Accession: 003704830

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