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Detection of dengue viral RNA in patients' sera by nucleic acid sequence-based amplification (NASBA) and polymerase chain reaction (PCR)


Detection of dengue viral RNA in patients' sera by nucleic acid sequence-based amplification (NASBA) and polymerase chain reaction (PCR)



Dengue Bulletin 26: 131-139



Nucleic acid sequence-based amplification (NASBA) was employed with a set of universal primers and probe based on the 3' non-coding region of the dengue viral RNA sequence. NASBA was used for the detection of the viral RNA in sera of patients clinically diagnosed as having dengue virus infection, and compared with polymerase chain reaction (PCR).

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